Coding

Part:BBa_K1499250:Design

Designed by: Raman Nelakanti   Group: iGEM14_StanfordBrownSpelman   (2014-08-27)

GFP (E0040) with two amber stop codons


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

This GFP was designed with two TAG stop codons in place of leucine residues to only be expressed correctly in Amberless cells.

References

Lajoie, MJ et al. (2013) Genomically Recoded Organisms Impart New Biological Functions. Science 342: 357-60. 24136966